Journal: Frontiers in Immunology
Article Title: The FGFR inhibitor Rogaratinib reduces microglia reactivity and synaptic loss in TBI
doi: 10.3389/fimmu.2024.1443940
Figure Lengend Snippet: FGFR inhibitor suppresses immune-related proteome signature at 3d post injury. (A) Outline of the experimental design; BAY121 was administered 30 mins after trauma and continued for 3d (25 mg/Kg once daily by oral gavage, vehicle alone as control). Samples were collected 3d post injury. (B) PCA plot of proteomic data shows the separation of TBI-Veh from BAY121-treated samples. (C, D) After modified differential protein expression analysis (FDR <0.05), subsets of upregulated (Red) and downregulated (Blue) proteins with log fold change and individual significance for (D) TBI-Veh compared to Sham–Veh and (D) TBI-BAY121 compared to TBI-Veh (n=5/group). (E) Protein-protein-interaction analysis revealed distinct clustering of downregulated and upregulated proteins in TBI-BAY121 vs TBI Veh; the cluster of downregulated proteins is enriched with immune- and inflammation-related proteins.
Article Snippet: Proteome Profiler Mouse Phospho-RTK Array Kit (R&D Systems, Minneapolis) was used to determine the Phospho RTK activation pattern.
Techniques: Control, Modification, Expressing